The Effect of Selected Biologically Active Compounds on the Expression of Fructose - 1,6 - Bisphosphate (FBPase) in H9C2 Cell Line and Culture Condition

Introduction: Fructose-1,6-bisphosphatase (FBPase) a gluconeogenic enzyme accumulates in the nucleus of proliferating cells, however its physiological role is not elucidated. In heart muscle cells localize on the Z-line, in the nuclei and in the intercalated discs. Accumulation of FBPase on the Z-line is important for proceeding of glyconeogenesis – the synthesis of glycogen from noncarbohydrate precursors like lactate. Recently employing neonatal rat cardiomyocytes in culture we have shown that FBPase localization on the Z-line is regulated in a calcium dependent manner. Aim of the study: In this study, we checked the effect of selected biologically active compound and culture conditions on FBPase localization in H9C2 cell line. Methods: H9C2 cells form embryonic rat heart were used for incubation in the presence of biologically active compounds: insulin, dantrolene and glucose. FBPase localization was examined by immunofluorescence and confocal microscopy. Results: We have found that FBPase in the H9C2 cells is localized in the cytoplasm and, in opposite to the neonatal rat cardiomyocytes in culture, in cell nuclei. Such localization was not affected by any of the stimulation and culture condition. Conclusions: These finding suggest influence on the glyconeogenesis regulation in cells obtained from the heart at different stages of development.